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Thursday, July 12, 2007

Answers to 1st term Test

Universal Science College Biochem 323 Plant Biochemistry July 9, 2007

Name of the student :

1. The cyclic electron flow during photosynthesis in plants does not result into (a) production of NADPH, (b) Production of ATP (c) Production of protons (d) none

2. The species having the highest reduction potential is (a) [ Mn – complex ] +4 (b) P680·+ (c) NADPH (d) H2O

3. During plant photosynthesis concentration of protons increases in (a) stroma (b) lamelle (c) chloroplast (d) lumen

4. In photosynthesis by purple bacteria which step is the slowest ? (a) charge separation (b) electron transfer from QA to QB (c) electron transfer from Cyt bc1 to (Chl)2·+ (d) electron transfer from pheophytin to QA

5. In photosynthesis photons raise __________ of electrons (a) reduction potential (b) oxidation potential (c) flow (d) none

6. Oxidation potential of DCPIP is ______ than water (a) higher (b) lower (c) same (d) none

7. Which one is not oriented towards lumen side ? (a) OEC (b) PC (c) Cytf (d) Cyt b6

8. Which sequence of steps can give ATP ? ( A – addition of pH 4 medium , B – removal of the medium by centrifugation , C – addition of pH 8 medium, D – addition of ADP + Pi , E – incubation for 2 hours )

(a) Chloroplast – C – E - B – D – A , (b) Chloroplast - A - E – B – C – D (c) Chloroplast – C – E - B – A – D and (d) Chloroplast - A - B – E – C – D

9. ATP synthase is located in (a) granal lamelle (b) stromal lamelle (c) lumen (d) stroma

10. Which statement is not true about OEC ? (a) It catalyzes the evolution of oxygen (b) It is oriented towards stromal side (c) It provides 4 electrons to PSII (d) It acts as a proton pump.

11. Which species accepts electrons from water in OEC ? (a) Tyr (b) [ Mn – complex ] 0

(c) [ Mn – complex ] +4 (d) P680·+

12. How many NADPH molecules are produced for each molecule of oxygen evolved ?

(a) 1 (b) 2 (c) 3 (d) 4

13. How many molecules of ATP are produced for each oxygen molecule evolved in photosynthesis ?

(a) 1 (b) 2 (c) 3 (d) 4

14. Under which condition electron flow during plant photosynthesis can become cyclic ?

(a) high [NADP] / [NADPH] (b) low [NADP] / [NADPH] (c) In presence of DCMU (d) In presence of DCPIP

15. Which of the following is not involved in Calvin cycle ?

(a) Ribose-5-P (b) G-3-P (c) Ribulose-1-P (d) Erythrose-4-P

16. In 4 Calvin cycles the number of Ribulose-1,5-BP formed is
(a) 0 (b) 1 (c) 2 (d) all wrong

17. During sucrose biosynthesis the direction of Pi transport by Pi – TP antiporter is
(a) stroma to cytosol (b) lumen to stroma (c) cytosol to stroma (d) all

18. Which enzymatic step ( excluding transport ) in photorespiration utilizes THF as the cofactor ?
(a) 4th (b) 5th (c) 6th (d) 7th

19. Which enzyme involved in CO2 fixation in C4 plants is not regulated by light ?
(a) Malate dehydrogenase (b) Malic enzyme (c) PEP carboxylase (d) Pyr phosphate dikinase

20. The pathway of CO2 fixation in a C4 plants has ____ enzymatic steps
(a) 4 (b) 5 (c) 6 (d) 7
( The rubisco reaction has been counted )

21. Which enzyme catalyzes the liberation of CO2 in a C4 plant ?
(a) Malate dehydrogenase (b) Malic enzyme (c) PEP carboxylase (d) Pyr phosphate dikinase

22. In photorespiration fixation of 2 molecules of O2 gives _____ molecules of C3 .
(a) 1 (b) 2 (c) 3 (d) 4;

23. The most abundant protein in mitochondria of a spinach plant is
(a) PEP carboxylase (b) glycine decarboxylase (c) Rubisco (d) None

24. In photorespiration Calvin cycle is gradually slowed down because of decreasing supply of
(a) NADPH (b) ATP (c) RuBP (d) b + c

25. Regeneration of RuBP from TP in stage 3 of Calvin cycle is accomplished in ___ distinct steps.
(a) 7 (b) 8 (c) 9 (d) 10

26. Which statement is not true about the effect of photosynthesis?
(a) Mg++ concentration increases in stroma (b) Mg++ concentration decreases in stroma (c) H+ concentration decreases in stroma (d) All

27. Which of the followings is not different for C3 and C4 plants ?
(a) carbon intermediates (b) ATP consumed (c) specificity for O2 (d) Calvin cycle

28. For C4 plants which statement is more accurate ?

(a) CO2 fixation and Calvin cycle are spatially separated (b) CO2 capture and Calvin cycle are spatially separated ( CO2 is captured in mesophyll cells and fixed in Calvin cycle in bundle sheath.)

29.Which statement is false about cyclic photophosphorylation ?
(a) ATP is produced (b) NADPH is not produced (c) Oxygen is evolved (d) all

30. When chloroplast is illuminated with a light of 700 nm, which of the following gets reduced ?

(a) Plastoquinone (b) Plastocyanin (c) Ferrodoxin (d) Pheophytin

31. Which contains only cyclic photosynthesis ? (a) Plants (b) Purple bacteria (c) green sulfur bacteria (d) All wrong

32. The reaction catalyzed by Malic enzyme is analogous to the one catalyzed by __________ enzyme.
(a) isocitrate dehydrogenase (b) α – ketogluatarate ddehydogenase (c) succinate dehydrogenase (d) none [ Notice the structural similarity of the portion undergoing decarboxylation ]

33. Starch biosynthesis is stimulated by (a) high level of Pi and low level of triose phosphate

(b) low level of Pi and high level of triose phosphate (c) high level of Pi and high level of triose phosphate (d) low level of Pi and low level of triose phosphate .

34. What will happen upon addition of DCPIP to chloroplast treated with DCMU herbicide ?
(a) Synthesis of ATP will be restored. (b) Evolution of oxygen will resume (c) NADPH production will not be restored (d) All wrong

35. Iodoacetate can inhibit an enzyme by reacting with thiol group in its active site. Which enzyme is not inhibited by iodoacetate ? (a) Malate dehdrogenase (b) Ribulose – 5 – phosphate kinase

(c) Rubisco (d) PEP carboxylase

36. In the activation of ____________ enzyme thioredoxin is involved

(a) glycine decarboxylase (b) Malic enzyme (c) F - 1,6 - bisphosphatase (d) Rubisco

37. A defect in sedoheptulose-1,7- bisphosphatase will totally inhibit the synthesis of (a) ribulose-5- phosphate (b) ribose-5-phosphate (c) xylulose-5-phosphate ( d) Erythrose-4-phosphate

38. In photophosphorylation __________ donates electrons (a) NADPH (b) NADH (c) H2O (d) PSII.

39. Efficiency of photosynthesis would be maximum, when chloroplast is illuminated with light of _____ nm. (a) 680 nm (b) 700nm (c) a + b (d) all wrong

40. Which statement about photosynthesis is not true ? (a) Dark reaction does not occur in light (b) Dark reaction does not occur in dark (c) Dark reaction occurs in both light and dark (d) None

41. Decrease in wavelength of light in photosynthesis will favor (a) stacking of grana (b) unstacking of grana (c) a + b (d) all wrong

42. Which intermediate is not involved in the reaction catalyzed by rubisco ?
(a) endioate (b) 3-PG (c) β – hydroxyl acid (d) carbanion

43. Rubisco enzyme is activated by (a) RuBP (b) CO2 (c) a + b (d) all wrong

44. The main reason for photorespiration is (a) high temperature (b) high O2 levels (c) rubisco possesses oxygenase activity (d) None

45. A defective Malic enzyme can inhibit CO2 fixation in (a) C4 plants (b) C3 plants (c) a + b (d) None

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